Pumpkin byproducts exhibit antioxidant and antimicrobial exercise

In a current research revealed in Molecules, researchers characterised the bioactive brokers within the meals trade byproducts of pumpkins.

Research: Organic Exercise of Pumpkin Byproducts: Antimicrobial and Antioxidant Properties. Picture Credit score: EvgeniiAnd/Shutterstock

Pure merchandise are more and more studied as sources of bioactive compounds. Nevertheless, vegetable-based meals are restricted to options comparable to ready-to-use meals in trendy societies. With a rising demand for these meals merchandise, a considerable amount of byproducts is produced within the meals trade and discarded alongside the method.

Analysis has not too long ago been centered on selling sustainability and exploring high-value compounds. As an example, extracts from candy potato leaves, primarily phenolic compounds, have been attributed with antioxidant, anti-diabetic, anti-mutagenic, anti-cancer, anti-inflammatory, cardioprotective, and hepatoprotective properties.

The manufacturing of formulations from pumpkin pulp is widespread within the meals trade, producing a excessive amount of peels, fibers, and seeds as byproducts. Though the pumpkin pulp is appreciated, its byproducts, which can include polyphenols, polyunsaturated fatty acids, minerals, and carotenoids, are under-utilized or discarded.

Concerning the research

Within the current research, researchers evaluated the byproducts of Algerian and Portuguese pumpkins to determine and characterize bioactive compounds.  Gold nugget pumpkin, Musquée de Provence, butternut squash pumpkins from native Algerian markets, and kabocha squash, widespread pumpkin, and butternut squash from native Portuguese markets have been obtained.

Samples (of fibrous strands, seeds, and peels) have been produced by segregating pulp from byproducts. These samples have been lyophilized, crushed, and extracted by maceration. The phenolic composition was decided utilizing high-performance liquid chromatography (HPLC) coupled to a diode array detector (DAD) and electrospray ionization/mass spectrometry (ESI/MS).

The antioxidant potential was assessed utilizing the oxidative hemolysis inhibition assay (OxHLIA) in sheep purple blood cells and the thiobarbituric acid reactive substance (TBARS) assay in porcine mind homogenates. Antimicrobial exercise was investigated towards micro organism (Yersinia, Staphylococcus, Escherichia, Pseudomonas, Enterobacter, Listeria, and Bacillus spp.) and fungi (Aspergillus spp.) by the p-iodonitrotetrazolium chloride technique. Cytotoxicity was examined by sulforhodamine B (SRB) colorimetric assay in major non-tumor porcine liver cells.


The authors recognized eight (tentative) compounds from pumpkin byproducts that belonged to the flavonoid, flavan-3-ol, and phenolic acid households. Flavonoids have been probably the most ample compounds detected, together with the O-glycosylated derivatives of quercetin, isorhamnetin, and kaempferol. The peel of widespread pumpkin (from Portugal) had statistically greater portions (9.4 mg/g) of phenolic compounds, adopted by kabocha squash fibers (4.8 mg/g) and butternut squash peel (4.73 mg/g).

Epicatechin was probably the most ample compound. In Algerian pumpkins, most phenolic acids have been recognized within the fibrous strands of gold nugget pumpkin, and flavonoids have been the very best within the peels and seeds of Musquée de Provence. The gold nugget pumpkin peels and fibers had the very best complete phenolic compounds at 4.1 mg/g and three.93 mg/g, respectively.

The extracts from Portuguese kabocha and butternut squash seeds confirmed the very best antioxidant exercise within the TBARS assay, with half-maximal inhibitory focus (IC50) values of 164 μg/mL and 185 μg/mL, respectively. The antioxidant exercise was not considerably completely different from that of Trolox (optimistic management) with an IC50 of 139 μg/mL.

The findings in OxHLIA have been comparable amongst samples, with IC50 values starting from 43 μg/mL to 96 μg/mL, roughly two to four-fold greater than for Trolox. Furthermore, Portuguese pumpkin samples had higher anti-hemolytic exercise than Algerian samples. Curiously, regardless of missing anti-hemolytic exercise, the extracts from gold nugget pumpkin seeds exhibited probably the most potent inhibition within the TBARS assay.

All Portuguese pumpkin samples confirmed inhibitory exercise towards Y. enterocolitica, whereas these from Algeria inhibited S. aureus. All samples inhibited the expansion of A. brasiliensis, whereas extracts from the fibrous strands of Algerian pumpkins have been protecting towards A. fumigatus. No pattern was cytotoxic to porcine liver cells, even on the most examined focus of 400 μg/mL.


In abstract, the research characterised the bioactive compounds from pumpkin byproducts. The butternut squash extracts from each international locations had probably the most potent antioxidant exercise, whereas gold nugget pumpkin seeds and butternut squash fibers confirmed the very best antimicrobial exercise. The widespread pumpkin from Portugal had the very best and most various profile of phenolic compounds. Total, the findings corroborate the importance of recycling meals trade byproducts to be used in numerous industries, comparable to cosmetics and pharmaceutics.

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